𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning of the integration and attachment regions of bacteriophage P4

✍ Scribed by Pierson, Leland S. ;Kahn, Michael L.

Publisher
Springer
Year
1984
Tongue
English
Weight
904 KB
Volume
195
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

The integration and attachment regions of bacteriophage P4 have been cloned into a multicopy plasmid. This plasmid can integrate into the E. coli chromosome at the same location as the parent phage. Integration increases the stability of the plasmid and allows it to be retained even under conditions in which a non-integrated plasmid would be lost. None of the genes needed for P4 lytic growth is required for integration. The P4 integration and attachment regions have been cloned on separate plasmids. A plasmid that carries the attachment site can integrate into the chromosome only if another plasmid that carries the P4 integration functions is present. A plasmid that carries only this trans-acting integration function cannot integrate. Using deletion mutants of the plasmid, the maximum size of the region needed for integration has been determined to be 1.6 kb, of which no more than 1.2 kb codes for the integrase protein. A nonsense mutant defective in integration has been isolated by using a rapid screening procedure that identifies unstable plasmids.

πŸ“œ SIMILAR VOLUMES

Assembly and attachment of bacteriophage
Assembly and attachment of bacteriophage T4 tail fibers
✍ Bishop, R. J. ;Conley, M. P. ;Wood, W. B. πŸ“‚ Article πŸ“… 1974 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 315 KB

## Abstract Bacteriophage T4 tail fibers are rodlike structures with a contour length of about 1400 Γ…, a diameter of about 45 Γ…, and a total mass of about 600,000 daltons. The assembly of the tail fibers and their subsequent attachment to the phage particle are under the control of 8 phage‐induced

Properties and products of the cloned in
Properties and products of the cloned int gene of bacteriophage P2
✍ Ljungquist, Elisabeth ;Bertani, L. Elizabeth πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 901 KB

Fragments of DNA of the temperate phage P2, generated by treatment with the restriction enzyme PstI, have been cloned into the plasmid pBR322. One such fragment, which has its endpoints within phage genes T and C, carries the structural P2 int gene as well as its promoter and the phage att site. Whe

Cloning of the immunity repressor determ
Cloning of the immunity repressor determinant of bacteriophage P2 in the pBR322 plasmid
✍ WestΓΆΓΆ, Anna ;Ljungquist, Elisabeth πŸ“‚ Article πŸ“… 1980 πŸ› Springer 🌐 English βš– 761 KB

Through in vitro recombination of DNA restriction fragments, we have constructed a plasmid, which expressed in vivo the immunity repressor gene (C) of bacteriophage P2. A bacterial strain carrying such a plasmid showed a high level of P2 specific immunity. It was lysogenized normally by an infecting

Integration of bacteriophages λ and ⊘80
Integration of bacteriophages λ and ⊘80 in wild-type Escherichia coli at secondary attachment sites
✍ Kholodii, G. Ya. ;Mindlin, S. Z. πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 636 KB

The frequency of occurrence and the genetic structure of polylysogens were studied for phages lambda, phi 80 and lambda att80. In the case of lambda, frequency of polylysogenization is high (0.20 to 0.41) with a tandem integration of prophages at the primary att site (att lambda). With phi 80 and la