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Cloning of the HIS5 gene of Saccharomyces cerevisiae by yeast transformation

✍ Scribed by Satoshi, Harashima; Sidhu, Rajinder S.; Akio, Toh-e; Yasuji, Oshima


Book ID
121964701
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
603 KB
Volume
16
Category
Article
ISSN
0378-1119

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✍ FrΓΆhlich, Kai-U. ;Entian, Karl-D. ;Mecke, Dieter πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 605 KB

Carbon catabolite repression in yeast depends on catalytic active hexokinase isoenzyme PII ( Entian 1980a ). A yeast strain lacking hexokinase isoenzymes PI and PII was transformed, using a recombinant pool with inserts of yeast nuclear DNA up to 10 kbp in length. One hundred transformants for hexok