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Cloning of the glucose isomerase (D-xylose isomerase) and xylulose kinase genes of Streptomyces violaceoniger

✍ Scribed by Marcel, Thierry ;Drocourt, Daniel ;Tiraby, Gérard


Publisher
Springer
Year
1987
Tongue
English
Weight
672 KB
Volume
208
Category
Article
ISSN
0026-8925

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Glucose kinase in Streptomyces coelicolor has a molecular weight of about 110,000. In crude extracts, the enzyme exhibited apparent Km values of 0.20 mM for ATP, 0.27 mM for glucose, and 2.2 mM for the glucose analogue 2-deoxyglucose. Mutations (glk) to 2-deoxyglucose-resistance, which greatly reduc