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Cloning of the gene coding for aromatic amino acid aminotransferase from anE. ColiB strain

✍ Scribed by Martyn Robinson; Rüdiger Marquardt; Johann Then; Janet McChesney; Hugh Neatherway; Gerhard Wöhner; Hans M. Deger; Paul Pràve

Publisher
Springer Netherlands
Year
1987
Tongue
English
Weight
399 KB
Volume
9
Category
Article
ISSN
0141-5492

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✍ Hoshino, Toshimitsu ;Kose, Kumiko ;Uratani, Yoshihiko 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 728 KB

The gene braB, encoding the Na+-coupled carrier for branched-chain amino acids in Pseudomonas aerugin- osa PAO, was cloned on cosmid pMMB34. The cosmid clones carrying the braB gene were identified as those that restored growth at low leucine concentration and Na+-dependent leucine transport activit