Cloning of the D-lactate dehydrogenase gene fromLeuconostoc mesenteroidessubsp.cremoris

✍ Scribed by V. Phalip; V. Dartois; P. Schmitt; C. Divies

• Publisher: Springer Netherlands
• Year: 1994
• Tongue: English
• Weight: 495 KB
• Volume: 16
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00134615

✦ Synopsis

A genomic library from Leuconostoc mesenteroides subsp. cremoris was screened for Dlactate dehydrogenase activity using a stereospecific lactate detection test on agar plate. Among 3500 clones tested, six posittve colonies were found on D-lactate detection plate, displaying significantly higher D-LDH activity than Escherichia coli host strain.