✦ LIBER ✦
Cloning of porcine IGF1 receptor cDNA and detection of sequence polymorphisms using RT-PCR
✍ Scribed by T. Harumi; K. Maruyama; H. Kagami; A. Sano; Y. Matsubara; T. Tagami; M. Naito
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 169 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0268-9146
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✦ Synopsis
A cDNA encoding the porcine type 1 insulin‐like growth factor receptor (IGF1R) was amplified by reverse transcriptase‐polymerase chain reaction (RT‐PCR). The sequence of a 4.2‐kb product was determined and had an open reading frame, encoding 1367 amino acids with 98.1 and 95.2% sequence similarity to the human and rat IGF1R, respectively. In the comparison of RT‐PCR derived IGF1R sequences from 12 unrelated pigs, 12 silent sequence variants were found.