Cloning of human telomeres by complementation in yeast
β Scribed by Cross, Sally H.; Allshire, Robin C.; McKay, Stewart J.; McGill, Nio I.; Cooke, Howard J.
- Book ID
- 109758702
- Publisher
- Nature Publishing Group
- Year
- 1989
- Tongue
- English
- Weight
- 515 KB
- Volume
- 338
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/338771a0
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π SIMILAR VOLUMES
A cDNA encoding farnesyl diphosphate synthase, an enzyme that synthesizes C15 isoprenoid diphosphate from isopentenyl diphosphate and dimethylallyl diphosphate, was cloned from an Arabidopsis thaliana cDNA library by complementation of a mutant of Saccharomyces cerevisiae deficient in this enzyme. T
Both insect and mammalian genes have previously been cloned by genetic complementation in yeast. In the present report, we show that the method can be applied also to plants. Thus, we have cloned a rape cDNA for 3-isopropylmalate dehydrogenase (IMDH) by complementation of a yeast leu2 mutation. The