Cloning of human erythroid progenitors (BFU-E) in the absence of fetal bovine serum
β Scribed by Giovanni Migliaccio; Anna Rita Migliaccio
- Book ID
- 114710999
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 437 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0007-1048
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## Abstract BFUβE from the blood of 14 normal adults have been grown by the plasma clot technique. The hemoglobins synthesized in burst colonies were purified from other proteins by affinity chromatography on Sepharoseβhaptoglobin. The radioactivity incorporated in the globin chains was estimated b
## New lersey 07 I03 Previously, we demonstrated that removal of fetal bovine serum (FBS) from the medium of human skin fibroblasts resulted in an accelerated '%b' washout, decreased cellular K i , and increased Na' contents. In the present study we examined the mechanism underlying these changes.