𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning of genes involved in negative regulation of production of extracellular enzymes and polysaccharide ofXanthomonas campestrispathovarcampestris

✍ Scribed by Ji-Liang Tang; Clare L. Gough; Michael J. Daniels

Publisher
Springer
Year
1990
Tongue
English
Weight
443 KB
Volume
222
Category
Article
ISSN
1617-4615

No coin nor oath required. For personal study only.

✦ Synopsis

A recombinant plasmid pIJ3079 contains DNA sequences from Xanthomonas campestris pv campestris involved in coordinate negative regulation of production of the extracellular enzymes protease, endoglucanase, amylase and polygalacturonate lyase, and extracellular polysaccharide (EPS). Wild-type bacteria harbouring pIJ3079 and therefore carrying extra copies of the gene(s) therein showed reduced enzyme and EPS production and reduced aggressiveness to plants. Localised Tn5 mutagenesis of the corresponding region of the genome gave mutants producing higher levels of enzymes and EPS than the wild type, suggesting that the gene(s) may negatively regulate production in the normal cell. Enzyme and EPS production in the mutants was still dependent on previously characterised positive regulatory genes.

📜 SIMILAR VOLUMES

Gene expression analysis of BCR/ABL1-dep
Gene expression analysis of BCR/ABL1-dependent transcriptional response reveals enrichment for genes involved in negative feedback regulation
✍ Petra Håkansson; Björn Nilsson; Anna Andersson; Carin Lassen; Urban Gullberg; Th 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 881 KB

## Abstract Philadelphia (Ph) chromosome‐positive leukemia is characterized by the BCR/ABL1 fusion protein that affects a wide range of signal transduction pathways. The knowledge about its downstream target genes is, however, still quite limited. To identify novel BCR/ABL1‐regulated genes we used

Crystal structure of the novel PaiA N-ac
Crystal structure of the novel PaiA N-acetyltransferase from Thermoplasma acidophilum involved in the negative control of sporulation and degradative enzyme production
✍ E.V. Filippova; L. Shuvalova; G. Minasov; O. Kiryukhina; Y. Zhang; S. Clancy; I. 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 711 KB

## Abstract GCN5‐related __N__‐acetyltransferases (GNATs) are the most widely distributed acetyltransferase systems among all three domains of life. GNATs appear to be involved in several key processes, including microbial antibiotic resistance, compacting eukaryotic DNA, controlling gene expressio