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Cloning of genes encoding coli-surface (CS) antigens in enterotoxigenic Escherichia coli

✍ Scribed by Geraldine A. Willshaw; Henry R. Smith; Moyra M. McConnell; Bernard Rowe


Book ID
109314342
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
425 KB
Volume
49
Category
Article
ISSN
0378-1097

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The pepN gene, that encodes aminopeptidase N in Escherichia coli, has been cloned into the multicopy plasmid pBR322. Expression of the cloned pepN gene results in overproduction of the enzyme. The restriction map of the 6.7 Kb insert was established and the gene was further localized by analysis of