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Cloning of DNA sequences involved in overproduction of endoglucanase activity inStreptomyces lividans66

✍ Scribed by F. Shareck; F. Mondou; R. Morosoli; D. Kluepfel

Publisher
Springer Netherlands
Year
1987
Tongue
English
Weight
380 KB
Volume
9
Category
Article
ISSN
0141-5492

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Spontaneous chloramphenicol-sensitive mutants of Streptomyces lividans 66 had previously been shown to be very unstable and to yield arginine auxotrophic mutants at a frequency of 25% of spores; the Arg-mutants had amplified a particular 5.7 kb DNA sequence to over one hundred tandem copies per geno