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Cloning of an alkaline phosphatase gene from the moderately thermophilic bacteriumMeiothermus ruberand characterization of the recombinant enzyme

โœ Scribed by J.V. Yurchenko; A.V. Budilov; S.M. Deyev; I.S. Khromov; A.Y. Sobolev


Publisher
Springer
Year
2003
Tongue
English
Weight
646 KB
Volume
270
Category
Article
ISSN
1617-4615

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โœ Won Hee Jang; Eun Kyung Kim; Hwanghee Blaise Lee; Jae Hoon Chung; Ook Joon Yoo ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 877 KB

A gene, aprP, encoding an extracellular alkaline serine protease from a newly isolated Pseudomonas sp. KFCC 10818 was cloned and characterized. Nucleotide sequence analysis revealed an open reading frame of 1,266 nucleotides which could encode a polypeptide comprised of 422 amino acids. The C-termin