Cloning of a tellurite resistance determinant from Bacillus stearothermophilus V in Escherichia coli
✍ Scribed by Claudio Vásquez; Claudia Saavedra; Claudia Loyola; Hugo Moscoso; Sergio Pichuantes
- Book ID
- 111743013
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 233 KB
- Volume
- 47
- Category
- Article
- ISSN
- 1521-6543
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A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII
Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferas