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Cloning of a haemolysin from Citrobacter freundii and its expression in phylogenetically related bacteria

✍ Scribed by Ali Al-Zaag; J.M. Pemberton

Book ID: 109313557
Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 245 KB
Volume: 37
Category: Article
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1986.tb01821.x

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✦ Synopsis

The determinants for a haemolysin from an extraintestinal isolate of Citrobacter freundii have been cloned and expressed in both Escherichia coli K12 and phylogenetically related bacteria. Compared with E. coli, where the haemolytic determinants are encoded in 7.5 kb, the hemolysin determinants of C. freundii are located on a 2.5-kb HindlII fragment in the recombinant plasmid PJP71. Chicken embryo tests indicate that this haemolysin does contribute to the pathogenicity of C. freundii.

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