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Cloning of a cDNA encoding a putative cysteine protease from germinating maize seeds

✍ Scribed by Everaldo G. deBarros; Brian A. Larkins

Book ID
119137369
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
947 KB
Volume
99
Category
Article
ISSN
0168-9452

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PCR cloning and expression analysis of c
PCR cloning and expression analysis of cDNAs encoding cysteine proteinases from germinating seeds ofVicia sativaL.
✍ Claudia Becker; JΓΌrgen Fischer; Van Hai Nong; Klaus MΓΌnitz πŸ“‚ Article πŸ“… 1994 πŸ› Springer 🌐 English βš– 756 KB

cDNA clones encoding cysteine proteinases from cotyledons of germinated seeds of Vicia sativa L. have been obtained by means of PCR. Degenerate oligonucleotide primers were designed according to conserved amino acid regions of known cysteine proteinases. The deduced amino acid sequences of the cDNA