Cloning of a breakpoint cluster region at band 3q27 involved in human non-Hodgkin's lymphoma

✍ Scribed by Clotilde Deweindt; Jean-Pierre Kerckaert; Hervé Tilly; Sabine Quief; Van Cong Nguyen; Christian Bastard

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 472 KB
• Volume: 8
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870080303

✦ Synopsis

In a previous cytogenetic analysis, we showed the recurrence of translocations involving band 3q27 and immunoglobulin gene regions in 20 out of 319 patients with non-Hodgkin's lymphoma (NHL). We report here the molecular cloning of the translocation breakpoint from tumor cells of a patient (LAR) with t(3; 14)(q27;q32) and the isolation of DNA probes which identify a major translocation cluster region (MTC) at band 3q27. A DNA library from LAR tumor cells was screened with a JH probe and several clones were identified corresponding either t o a somatic rearrangement of I G H genes (V4-D2-J6-Cp clonal rearrangement) or t o the t(3;14). Analysis of the t(3; 14) breakpoint showed that chromosome 3 material was translocated to an inverted 14q32 VH-containing fragment which was itself translocated to the J3 gene. Chromosome 3-assigned probes were used t o investigate local DNA rearrangements in a series of NHL with 3q27 translocations. Rearrangements were detected in I 3 of I 7 patients including 9 of I I with t(3; 14)(q27;q32), I of 2 with t(2;3)(p 12;q27), I of 2 with t(3;22)(q27;q I I), and 2 of 2 NHL with translocations not involving an IG gene, namely, t(3;4)(q27;pl I) and t(3;7)(q27;pl2). The finding of this MTC should be useful for diagnostic and prognostic studies and for the identification of a novel oncogene at band 3q27 involved in the development of B cell NHL. Genes Chrom Cancer 8:149-154 (1993).