Cloning, nucleotide sequence determination and expression of the genes encoding cytochrome P-450soy (soyC) and ferredoxinsoy (soyB) from Streptomyces griseus

Cloning sequencing and expression of the

Cloning sequencing and expression of the gene for cytochrome P450meg, the steroid-15β-monooxygenase from Bacillus megaterium ATCC 13368

✍ Rauschenbach, Reimund ;Isernhagen, Marina ;Noeske-Jungblut, Christiane ;Boidol, 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 839 KB

A 4.3 kb EcoRI fragment carrying the gene for cytochrome P450meg, the steroid-15 beta-monooxygenase from Bacillus megaterium ATCC 13368, was cloned and completely sequenced. The gene codes for a protein of 410 amino acids and was expressed in Escherichia coli and B. subtilis. Protein extracts from t