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Cloning, nucleotide sequence, and expression of the Clostridium thermocellum cellodextrin phosphorylase gene and its application to synthesis of cellulase inhibitors

โœ Scribed by Takashi Kawaguchi; Yasuo Ikeuchi; Noriko Tsutsumi; Akihiko Kan; Jun-Ichi Sumitani; Motoo Arai


Book ID
114294249
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
925 KB
Volume
85
Category
Article
ISSN
0922-338X

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The gene coding for 3-phosphoglycerate kinase (PGK) in ML-236B (compactin)-producing Penicillium citrinum was isolated from the recombinant phage lambda library using the corresponding Aspergillus nidulans pgk gene as a probe. The P. citrinum pgk gene has an open reading frame of 1,254 bp, encoding