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Cloning, expression, purification, and characterization of Vibrio cholerae transcriptional activator, HlyU

โœ Scribed by Rudra Prasad Saha; Gautam Basu; Pinak Chakrabarti


Book ID
116828356
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
515 KB
Volume
48
Category
Article
ISSN
1046-5928

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A library containing more than 80% of the Vibrio cholerae genome was constructed by cloning BamH1 restriction fragments into pBR322. Using interspecific complementation of an Escherichia coli recA mutant with plasmids containing the gene bank of V. cholerae, a recA-like gene was identified. The reco