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Cloning, Expression, Purification, and Characterization of Cold-Adapted α-Amylase fromPseudoalteromonas arcticaGS230

✍ Scribed by Mingsheng Lu; Shujun Wang; Yaowei Fang; Huangzhong Li; Shu Liu; Hongfei Liu


Book ID
106423970
Publisher
Springer
Year
2010
Tongue
English
Weight
344 KB
Volume
29
Category
Article
ISSN
1573-4943

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The alpha-amylase precursor from the bacterium Pseudoalteromonas haloplanktis possesses a propeptide at the C-terminus possibly responsible for outer membrane translocation. Unlike the predicted beta-barrel of autotransporters, this C-terminal propeptide displays a noticeable alpha-helix content. It