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Cloning, expression, and purification of glyoxysomal 3-oxoacyl-CoA thiolase from sunflower cotyledons

✍ Scribed by Anke C Schiedel; Silke Oeljeklaus; Patricia Minihan; James H Dyer


Book ID
116827730
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
973 KB
Volume
33
Category
Article
ISSN
1046-5928

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Simultaneous single-step purification of
✍ Mark X. Sliwkowski; Maris G.N. Hartmanis πŸ“‚ Article πŸ“… 1984 πŸ› Elsevier Science 🌐 English βš– 795 KB

Thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri were purified by ion-exchange chromatography to near homogeneity in a simultaneous, single-step procedure. The yield of both enzymes was greater than 80%. Thiolase was purified approximately 8-fold with sp act 1