Cloning and structure of a mouse interleukin-2 chromosomal gene

✍ Scribed by W. Degrave; G. Simons; R. Devos; G. Plaetinck; E. Remaut; J. Tavernier; W. Fiers

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 364 KB
• Volume: 11
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00417597

✦ Synopsis

Using non-stringent hybridization with a human interleukin-2 cDNA probe, we have isolated recombinant phages from a mouse genomic DNA library cloned in the EMBL3 phage. The sequence and organization of the mouse interleukin-2 (IL-2) gene was determined. By comparison with the human IL-2 sequence, three introns can be identified with lengths of 99, + 2 400, and + 1 900 base pairs, respectively. The mouse IL-2 gene codes for a polypeptide of 169 amino acids and contains a putative signal peptide of 20 amino acids. The homology to the human interleukin-2 is 72~ at the nucleotide level in the coding part and 65~ at the amino acid level. An extraordinary sequence, consisting of 12 consecutive CAG codons coding for glutamihe, is found in the first exon.