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Cloning and sequencing of the Klebsiella pneumoniae tonB gene and characterization of Escherichia coli-K. pneumoniae TonB hybrid proteins

✍ Scribed by Bruske, Anja K.; Anton, Martina; Heller, Knut J.


Book ID
123421981
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
936 KB
Volume
131
Category
Article
ISSN
0378-1119

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From a gene bank of Klebsiella pneumoniae M5a1, a 1.7 kb gene fragment was isolated which was able to restore the Ntr+ phenotype and ammonium (methylammonium) transport, but not glutamate synthase in an Escherichia coli glt mutant (glutamate synthase deficiency). The fragment strongly hybridized wit