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Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli

✍ Scribed by Carol E. Jones; Toni M. Fleming; Peter W. Piper; Jennifer A. Littlechild; Don A. Cowan


Book ID
116132715
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
302 KB
Volume
160
Category
Article
ISSN
0378-1119

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The entire structural gene for tyrocidine synthetase 1 from Bacillus brevis ATCC 8185 has been cloned and expressed in Escherichia coli. Transformed E. coli cells were screened for their ability to produce tyrocidine synthetase 1 by in situ immunoassay using antibodies against gramicidin S synthetas