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Cloning and sequencing of a cyclodextrin glycosyltransferase gene from Brevibacillus brevis CD162 and its expression in Escherichia coli

✍ Scribed by Myung Hee Kim; Cheon Bae Sohn; Tae Kwang Oh


Book ID
109322368
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
757 KB
Volume
164
Category
Article
ISSN
0378-1097

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The entire structural gene for tyrocidine synthetase 1 from Bacillus brevis ATCC 8185 has been cloned and expressed in Escherichia coli. Transformed E. coli cells were screened for their ability to produce tyrocidine synthetase 1 by in situ immunoassay using antibodies against gramicidin S synthetas