Cloning and sequencing of a cDNA encoding a copper-zinc superoxide dismutase enzyme from the marine yeastDebaryomyces hansenii

✍ Scribed by Hernández-Saavedra, Norma Y.; Egly, Jean Marc; Ochoa, José Luis

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 381 KB
• Volume: 14
• Category: Article
• ISSN: 0749-503X
• DOI: 10.1002/(sici)1097-0061(19980430)14:6<573::aid-yea244>3.0.co;2-8

✦ Synopsis

Cu-Zn superoxide dismutase (SOD-1) is a ubiquitously occurring eukaryotic enzyme with a variety of important effects on respiring organisms. A gene (dhsod-1) encoding a Cu-Zn superoxide dismutase of the marine yeast Debaryomyces hansenii was cloned using mRNA by the RT-PCR technique. The deduced amino-acid sequence shows approximately 70% homology with that of cytosolic superoxide dismutase from Saccharomyces cerevisiae and Neurospora crassa, as well as lower homologies (between 55 and 65%) with the corresponding enzyme of other eukaryotic organisms, including human. The gene sequence encodes a protein of 153 amino acids with a calculated molecular mass of 15-92 kDa, in agreement with the observed characteristics of the purified protein from D. hansenii.