Cloning and preliminary characterization of the streptothricin resistance determinants of the transposons Tn1825 and Tn1826

✍ Scribed by Dr. sc. nat. E. Tietze; J. Brevet; H. Tschäpe; W. Voigt

• Book ID: 102390931
• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 488 KB
• Volume: 28
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.3620280116

✦ Synopsis

Streptothricin resistance determinants have been cloned from the transposons Tn1825 and Tnl826 to the vector plasmid pUC8. The recombinant plasmids were characterized with respect to their physical structure. The resistance properties of their hosts were characterized with respect to the minimal inhibitory concentration of streptothricin and the activity of the streptothricin acetyltransferase. The proteins encoded by the cloned resistance determinants were analysed in a maxicell system. The results of our investigations suggest that the resistance to streptothricin is mediated by the activity of a streptothricin acetyltransferase with both, Tn 1825 and Tn1826. However, the resistancedeterminant ofTn1825 is morecompler than Tn1826 and additional functions involved in realizing the resistance phenotype must be recognized.