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Cloning and nucleotide sequences of the genes involved in the meta-cleavage pathway of cumene degradation in Pseudomonas fluorescens IP01

✍ Scribed by Hiroshi Habe; Toshiaki Kimura; Hideaki Nojiri; Hisakazu Yamane; Toshio Omori


Book ID
113397802
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
841 KB
Volume
81
Category
Article
ISSN
0922-338X

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The gene braB, encoding the Na+-coupled carrier for branched-chain amino acids in Pseudomonas aerugin- osa PAO, was cloned on cosmid pMMB34. The cosmid clones carrying the braB gene were identified as those that restored growth at low leucine concentration and Na+-dependent leucine transport activit