Cloning and nucleotide sequence of the mono- and diacylglycerol lipase gene (mdlB) of Aspergillus oryzae
โ Scribed by Atsushi Tsuchiya; Hidekazu Nakazawa; Jinichi Toida; Kunio Ohnishi; Junichi Sekiguchi
- Book ID
- 109321318
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 465 KB
- Volume
- 143
- Category
- Article
- ISSN
- 0378-1097
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๐ SIMILAR VOLUMES
Using synthetic oligonucleotide probes, we have cloned a genomic DNA sequence encoding a ribonuclease (RNase) T2 gene (rntB) from Aspergillus oryzae on a 4.8 kb HindIII fragment. DNA sequence analysis of the RNase T2 revealed the following: (1) The gene is arranged as five exons and four introns; (2
A lipase gene from B. subtilis WRRL-B558 was cloned in Escherichia coli JM109 using pBluescript as a vector plasmid. Two methods were combined to screen for the lipase-producing clone. The first was done by overlaying the screening plates with ฮฒ-naphthylacetate and Fast Blue BB dye. Positive clones