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Cloning and High-Level Expression of Recombinant Ara h 6

✍ Scribed by Charles, T.P.; Hurlburt, B.K.; Landry, S.J.; Maleki, S.J.

Book ID
119284297
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
41 KB
Volume
127
Category
Article
ISSN
1097-6825

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## Abstract We have expressed active human cathepsin S to 60 mg/L in Sf9 cells using a baculovirus system. Production of milligram quantities has facilitated crystallographic studies to determine the structure of this enzyme, which has unique properties among lysosomal cysteine proteinases. Recombi