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Cloning and expression of two cellulase genes of Clostridium cellulolyticum in Escherichia coli

✍ Scribed by Faure, Eric; Bagnara, Chantal; Belaich, Anne; Belaich, Jean-Pierre


Book ID
121774698
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
769 KB
Volume
65
Category
Article
ISSN
0378-1119

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DNA from Clostridium acetobutylicum ABKn8 was partially digested with Sau3A and the fragments obtained were inserted into the unique BamHI site of the cloning vector pHV33. The recombinant plasmids were used to transform Escherichia coli HB101 with selection for ampicillin resistance. A collection o