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Cloning and expression of the Rhodospirillum rubrum ribulosebisphosphate carboxylase gene in E. coli

✍ Scribed by Somerville, Christopher R. ;Somerville, Shauna C.


Publisher
Springer
Year
1984
Tongue
English
Weight
688 KB
Volume
193
Category
Article
ISSN
0026-8925

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A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'