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Cloning and expression of the penicillinase from a borderline methicillin-susceptible Staphylococcus aureus strain in Escherichia coli

โœ Scribed by Orietta Massidda; Maria Pia Montanari; Marina Mingoia; Pietro Emanuele Varaldo


Book ID
109320021
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
661 KB
Volume
119
Category
Article
ISSN
0378-1097

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Cloning of the tyrocidine synthetase 1 g
โœ Marahiel, Mohamed A. ;Krause, Michael ;Skarpeid, Hans-Jacob ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 907 KB

The entire structural gene for tyrocidine synthetase 1 from Bacillus brevis ATCC 8185 has been cloned and expressed in Escherichia coli. Transformed E. coli cells were screened for their ability to produce tyrocidine synthetase 1 by in situ immunoassay using antibodies against gramicidin S synthetas