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Cloning and expression of the lepidopteran toxin produced by Bacillus thuringiensis var. thuringiensis in Escherichia coli

โœ Scribed by Honigman, Alik; Nedjar-Pazerini, Galit; Yawetz, Aminadav; Oron, Uri; Schuster, Silvia; Broza, Meir; Sneh, Baruch


Book ID
121977531
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
956 KB
Volume
42
Category
Article
ISSN
0378-1119

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The gene (cytA) coding for the 27 kDa polypeptide of the Bacillus thuringiensis var. israelensis mosquito larvicidal delta-endotoxin, was cloned into a plasmid containing the T7 bacteriophage promoter. The plasmid was used to transform an Escherichia coli strain containing the T7 RNA polymerase gene