Cloning and expression of phospholipase D genepldfromStreptomyces chromofuscus
β Scribed by Bin Li; Fu-Ping Lu; Lin Tian; Yu Li; Lianxiang Du
- Publisher
- Springer-Verlag
- Year
- 2008
- Tongue
- English
- Weight
- 233 KB
- Volume
- 58
- Category
- Article
- ISSN
- 1590-4261
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## Abstract Toxin phospholipasesβD present in the venom of __Loxosceles__ spiders is the principal responsible for local and systemic effects observed in the loxoscelism. In this study, we describe the cloning, expression, functional evaluation, and potential biotechnological application of cDNAs,
The substrate specificity for phospholipase D from Streptomyces chromofuscus (PLD Sc ) has been determined utilizing an assay based on the quantitation of inorganic phosphate. 1,2-Di-n-hexanoyl phosphatidylcholine (C6PC), phosphatidylethanolamine (C6PE), phosphatidylserine (C6PS), phosphatidylglycer