Disruption of the KEX1 gene inPichia pas
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Boehm, Thomas; Pirie-Shepherd, Steven; Trinh, Loc-Ba; Shiloach, Joseph; Folkman,
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Article
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1999
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John Wiley and Sons
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English
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Endostatin is a potent angiogenesis inhibitor. In order to isolate sufficient quantities of soluble protein for in vivo studies in mice, we expressed murine endostatin in Pichia pastoris. Analysis of the expressed protein by mass spectrometry indicated that the protein was truncated. N-terminal sequ