Cloning and expression of a plant homologue of the small subunit of the Golgi-associated clathrin assembly protein AP19 fromCamptotheca acuminata

✍ Scribed by Ignacio E. Maldonado-Mendoza; Craig L. Nessler

• Book ID: 104619245
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 612 KB
• Volume: 32
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00041398

✦ Synopsis

Clathrin-coated vesicles (CCVs) are involved in selective protein transport in eukaryotes. AP-1 and AP-2 are protein complexes found in the CCVs of the Golgi apparatus and the plasma membrane respectively. AP19 is the smallest polypeptide chain components of AP-1. We have identified a cDNA clone (CAP19) encoding a putative homologue for the assembly protein API 9 from the Chinese medicinal tree, Camptotheca acuminata. The deduced polypeptide contains 161 amino acids and has a predicted Mr of 18 820. DNA blot analysis suggests that the AP19s of C. acuminata are encoded by a small gene family. CAPI9 was expressed ubiquitously throughout the plant suggesting that it may be involved in general Golgi-mediated secretion.