𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning and expression of a member of the Aspergillus niger gene family encoding α-galactosidase

✍ Scribed by den Herder, Irma F. ;Mateo Rosell, Ana M. ;van Zuilen, Caren M. ;Punt, Peter J. ;van den Hondel, Cees A. M. J. J.

Publisher
Springer
Year
1992
Tongue
English
Weight
897 KB
Volume
233
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

An enzyme with alpha-galactosidase activity and an apparent molecular weight of 82 kDa was purified from culture medium of Aspergillus niger. The N-terminal amino acid sequence of the purified protein shows similarity to the N-terminal amino acid sequence of alpha-galactosidases from several other organisms. Oligonucleotides, based on the N-terminal amino acid sequence, were used as probes to clone the corresponding gene from a lambda EMBL3 gene library of A. niger. The cloned gene (aglA) was shown to be functional by demonstrating that the 82 kDa alpha-galactosidase is absent from a strain with a disruption of the aglA gene, and is over-produced in strains containing multiple copies of the aglA gene. Enzyme activity assays revealed that the 82 kDa alpha-galactosidase A represents a minor extracellular alpha-galactosidase activity in A. niger.

📜 SIMILAR VOLUMES

Isolation and molecular characterisation
Isolation and molecular characterisation of the benzoate-para-hydroxylase gene (bphA) of Aspergillus niger: A member of a new gene family of the cytochrome P450 superfamily
✍ van Gorcom, Robert F. M. ;Boschloo, Johan G. ;Kuijvenhoven, Anneke ;Lange, Jacqu 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 766 KB

The gene coding for benzoate-para-hydroxylase (bphA) of Aspergillus niger was cloned using differential hybridisation techniques and complementation of mutants deficient in this enzyme activity. The nucleotide sequence of the gene was determined, the presence of two introns was shown and the transcr

Cloning and characterization of a Dictyo
Cloning and characterization of a Dictyostelium gene encoding a small GTPase of the Rab11 family
✍ Ioanna A. Dragoi; Theresa J. O'Halloran 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 184 KB 👁 2 views

Eukaryotic cells achieve complexity by compartmentalizing a subset of cellular functions into membranebound organelles. Maintaining this high level of cellular organization requires precise regulation of traffic between membranes. This task is accomplished, in part, by rab proteins. How these small

A member of the melanoma antigen-encodin
A member of the melanoma antigen-encoding gene (MAGE) family is expressed in human skin during wound healing
✍ Jürgen C. Becker; Reinhard Gillitzer; Eva-Bettina Bröcker 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 French ⚖ 428 KB 👁 1 views

MACE-I has been identified as a human gene, which directs the expression of an antigen being recognized on melanoma cells by autologous cytolytic T cells. MACE-I is expressed in melanomas and some other tumors. It has been proposed that this gene may be linked to the transformation event and therefo

Gene structure and spatiotemporal expres
Gene structure and spatiotemporal expression profile of tomato genes encoding YUCCA-like flavin monooxygenases: The ToFZY gene family
✍ Marino Expósito-Rodríguez; Andrés A. Borges; Andrés Borges-Pérez; José A. Pérez 📂 Article 📅 2011 🏛 Elsevier Science 🌐 English ⚖ 888 KB

The flavin monooxygenases (FMO) encoded by plant YUCCA genes are thought to catalyze a rate-limiting step in the tryptamine pathway for indole-3-acetic acid biosynthesis. Recent experiments with different plant models have indicate that YUCCA genes play essential roles in growth and development thro