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Cloning and Expression in Escherichia coli of Thermotoga neapolitana Genes Coding for Enzymes of Carbohydrate Substrate Degradation

โœ Scribed by O.N. Dakhova; N.E. Kurepina; V.V. Zverlov; V.A. Svetlichnyi; G.A. Velikodvorskaya


Book ID
115573353
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
279 KB
Volume
194
Category
Article
ISSN
0006-291X

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The 7.7 Mdal PstI fragment of Bacillus pumilus IPO containing genes for xylan degradation, xylanase, and fl-xylosidase was inserted at the Pstl site of pBR322 and cloned in E. coli C600. The hybrid plasmid thus formed was named pOXN29. The amount of xylanase and fl-xylosidase expressed in E. coli ha