𝔖 Bobbio Scriptorium
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Cloning and expression in Escherichia coli of Clostridium thermocellum genes coding for amino acid synthesis and cellulose hydrolysis

✍ Scribed by Philippe Cornet; Diana Tronik; Jacqueline Millet; Jean-Paul Aubert


Book ID
109312148
Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
304 KB
Volume
16
Category
Article
ISSN
0378-1097

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DNA from Clostridium acetobutylicum ABKn8 was partially digested with Sau3A and the fragments obtained were inserted into the unique BamHI site of the cloning vector pHV33. The recombinant plasmids were used to transform Escherichia coli HB101 with selection for ampicillin resistance. A collection o