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Cloning and expression in Escherichia coli of a recA-like gene from Bacteroides fragilis

✍ Scribed by Goodman, Heide J.K.; Parker, Joan R.; Southern, James A.; Woods, David R.


Book ID
123273689
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
676 KB
Volume
58
Category
Article
ISSN
0378-1119

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Cloning and expression in Escherichia co
✍ Paul, Kalidas ;Ghosh, Subrata K. ;Das, Jyotirmoy πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 645 KB

A library containing more than 80% of the Vibrio cholerae genome was constructed by cloning BamH1 restriction fragments into pBR322. Using interspecific complementation of an Escherichia coli recA mutant with plasmids containing the gene bank of V. cholerae, a recA-like gene was identified. The reco