Cloning and expression analysis of a G-protein subunit— in the rice water weevil lissorhoptrus oryzophilus Kuschel
✍ Scribed by Gui-Juan Kang; Zhong-Jun Gong; Jia-An Cheng; Cun-Gui Mao; Zeng-Rong Zhu
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 271 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0739-4462
No coin nor oath required. For personal study only.
✦ Synopsis
The open reading frame (ORF) encoding a novel G protein a subunit, Lo Ga o , was cloned from the parthenogenetic rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae). The Lo Ga o ORF encodes a protein of 354 amino acid residues. The deduced protein sequence shares high homology with Ga o from other species. The expression patterns of Lo Ga o in various adult tissues were indicated by real-time quantitative PCR and Western blot. The results showed that Lo Ga o mRNA was expressed at similar levels in tissues except relative high levels in the antennae of adult, and Lo Ga o protein of an apparent molecular mass of about 40 kDa was expressed in various tissues of the adult. Immunocytochemical localization showed that Lo Ga o was mainly expressed in the dendrites of the trichoid sensilla in the antenna of the weevil. The tissue and cellular localization of Lo Ga o suggests that Lo Ga o may take a part in signal transduction of olfactory/gustatory.