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Cloning and DNA sequence of the 5'-exonuclease gene of bacteriophage T5

✍ Scribed by A.V. Kaliman; A.I. Krutilina; V.M. Kryukov; A.A. Bayev

Book ID
115917760
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
409 KB
Volume
195
Category
Article
ISSN
0014-5793

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Bacteriophage T5 was subjected to combined hydrolysis with the restriction endonucleases PstI and HindIII and the resulting fragments were inserted into the plasmid pBR322. Selection of transformants for Aps-Tcr-phenotype made it possible to screen the hybrid plasmids that contained promoter sequenc