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Cloning and disruption of the yeast C-8 sterol isomerase gene

โœ Scribed by William H. Ashman; Robert J. Barbuch; Corinne E. Ulbright; Harry W. Jarrett; Martin Bard


Book ID
112774333
Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
640 KB
Volume
26
Category
Article
ISSN
0024-4201

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A recombinant plasmid was isolated from a Kluyveromyces lactis genomic DNA library which complements a Saccharomyces cerevisiae arg8 mutant defective in the gene encoding acetylornithine aminotransferase. The complementation activity was found to reside within a 2.0 kb DNA fragment. Nucleotide seque