The gene coding for 3-phosphoglycerate kinase (PGK) in ML-236B (compactin)-producing Penicillium citrinum was isolated from the recombinant phage lambda library using the corresponding Aspergillus nidulans pgk gene as a probe. The P. citrinum pgk gene has an open reading frame of 1,254 bp, encoding
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Cloning and characterization of two 3-phosphoglycerate kinase genes ofRhizopus niveusand heterologous gene expression using their promoters
โ Scribed by Naoki Takaya; Koji Yanai; Hiroyuki Horiuchi; Akinori Ohta; Masamichi Takagi
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 871 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0172-8083
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A gene encoding phosphoglycerate kinase (PGK) was isolated from the genomic library of C. maltosa to construct an expression vector for this yeast. The PGK gene had an open reading frame of 1 251 base pairs encoding approximately 47-kDA polypeptide of 417 amino-acid residues. Expression of this gene