Cloning and characterization of theSKI3gene ofSaccharomyces cerevisiaedemonstrates allelism toSKI5

✍ Scribed by Linda Hougan; David Y. Thomas; Malcolm Whiteway

• Publisher: Springer-Verlag
• Year: 1989
• Tongue: English
• Weight: 469 KB
• Volume: 16
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00391469

✦ Synopsis

We have identified a mutant strain of the yeast Saccharomyces cerevisiae which overproduces killer toxin. This strain contains a single mutation which fails to complement defects in both the SKI3 and SKI5 genes, while a cloned copy of this gene complements both the ski3 and ski5 defects. The level of secreted toxin from a cDNA based plasmid is not increased in a ski3 strain, showing that the overproduction phenotype is dependent upon an increased level of M1 dsRNA.