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Cloning and characterization of theHansenula polymorpha homologue of theSaccharomyces cerevisiae PMR1 gene

✍ Scribed by Kang, Hyun Ah; Kim, Jeong-Yoon; Ko, Su-Min; Park, Cheon Seok; Ryu, Dewey D. Y.; Sohn, Jung-Hoon; Choi, Eui-Sung; Rhee, Sang-Ki

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 228 KB
Volume: 14
Category: Article
ISSN: 0749-503X
DOI: 10.1002/(sici)1097-0061(19980930)14:13<1233::aid-yea322>3.0.co;2-y

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✦ Synopsis

A gene homologous to Saccharomyces cerevisiae PMR1 has been cloned in the methylotrophic yeast Hansenula polymorpha. The partial DNA fragment of the H. polymorpha homologue was initially obtained by a polymerase chain reaction and used to isolate the entire gene which encodes a protein of 918 amino acids. The putative gene product contains all ten of the conserved regions observed in P-type ATPases. The cloned gene product exhibits 60•3% amino acid identity to the S. cerevisiae PMR1 gene product and complemented the growth defect of a S. cerevisiae pmr1 null mutant in the EGTA-containing medium. The results demonstrate that the H. polymorpha gene encodes the functional homologue of the S. cerevisiae PMR1 gene product, a P-type Ca 2+ -ATPase. The DNA sequence of the H. polymorpha homologue has been submitted to GenBank with the Accession Number U92083.

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