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Cloning and characterization of the nuclear gene and cDNAs for triosephosphate isomerase of the marine red algaGracilaria verrucosa

✍ Scribed by Yi-Hong Zhou; Mark A. Ragan

Publisher: Springer-Verlag
Year: 1995
Tongue: English
Weight: 747 KB
Volume: 28
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00326429

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✦ Synopsis

cDNAs and an intronless single-copy nuclear gene (TPI1) encoding triosephosphate isomerase have been cloned and sequenced from the marine red alga Gracilaria verrucosa. The predicted amino-acid sequence of TPI1 is readily alignable with those of other known TPIs; 26 of 27 active-site residues and 19 of 26 intersubunit-contact residues are identical between TPIs of G. verrucosa and/or animals and green plants. A partial cDNA sequence of a second TPI gene (TPI2), presumably encoding plastid-localized TPI, was recovered by PCR and demonstrated by phylogenetic analysis to be red algal; no TP12 cDNA or genomic clones could be recovered. Genomic Southern analysis demonstrated that at least two TPI-like genes are present in the nuclear DNA of G. verrucosa.

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