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Cloning and characterization of the glutamine synthetase gene from Chinese shrimpFenneropenaeus chinensis

✍ Scribed by Xiao Fang Lai; Huan Gao; Jie Kong; Qing Yin Wang; Wei Ji Wang; Xian Hong Meng


Publisher
Springer Netherlands
Year
2011
Tongue
English
Weight
638 KB
Volume
19
Category
Article
ISSN
0967-6120

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Cloning and characterization of the prs
✍ Hove-Jensen, Biarne πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 865 KB

The gene, prs, encoding phosphoribosylpyrophosphate (PRPP) synthetase of Escherichia coli was isolated from a library of E. coli genes cloned in the bacteriophage 2D69 vector. A strain with a temperature-lethal defect in PRPP synthetase, (prs-2), was used as the host and cloning was performed by lys