Cloning and characterization of keratin D, a murine endodermal cytoskeletal protein induced during in vitro differentiation of F9 teratocarcinoma cells

✍ Scribed by Alonso, A. ;Weber, T. ;Jorcano, J. L.

• Book ID: 104747371
• Publisher: Springer-Verlag
• Year: 1987
• Tongue: English
• Weight: 910 KB
• Volume: 196
• Category: Article
• ISSN: 1432-041X
• DOI: 10.1007/bf00376018

✦ Synopsis

We have identified a eDNA coding for the routine keratin D from a collection of clones representing F9 teratocarcinoma stem cell mRNA sequences. These sequences are synthesized specifically after the addition of retinoic acid and cAMP to the culture medium. The clone is 1,382 nucleotides long and contains the entire information for the active polypeptide, the complete 3' end and most, if not all, of the 5' non-coding region. The mRNA is found in hepatocytes, in PYS-2 cells (an endodermal cell line) and in differentiated (retinoic-acid-treated) F9 cells, but not in untreated F9 cells. The length of the mRNA is 1.4 kb, as estimated by Northern blot hybridization. Southern hybridization performed under very stringent conditions detects a single fragment hybridizing strongly with the cloned eDNA, suggesting that the mouse genome contains only one or very few copies of this gene. We present the first complete sequence of a keratin expressed in simple epithelia, i.e. keratin D, and discuss its structural features.